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Construction of a universal recombinant expression vector that regulates the expression of human lysozyme

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 3,   Pages 382-389 doi: 10.15302/J-FASE-2018211

Abstract:

The mammary gland provides a novel method for producing recombinant proteins in milk of transgenicmethodology is expected to have the advantages of both plasmid and bacterial artificial chromosome (BACThe BAC of mouse whey acidic protein gene (mWAP) was modified twice by homologous recombinationpronuclei of fertilized mouse embryos, to successfully generate two transgenic mouse lines that expressed recombinant

Keywords： BAC recombinant methods     gene expression     human lysozyme     transgenic mice     milk expression vector    

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Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3,   Pages 179-184 doi: 10.15302/J-FASE-2014020

Abstract: In this study, we cloned the gene into a human lactoferrin (hLF) bacterial artificial chromosome (BAC) construct to make a hLF-hBCHE BAC construct.Subsequently, we injected the BAC construct into pronuclei of mouse fertilized embryos and generatedExpression analysis showed that recombinant hBCHE (rhBCHE) was expressed efficiently in the mammary gland

Keywords： recombinant human butyrylcholinesterase (rhBCHE)     human lactoferrin bacterial artificial chromosome (hLFBAC)     transgenic mice     milk    

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Development of pretreatment protocol for DNA extraction from biofilm attached to biologic activated carbon (BAC

Shuting ZHANG, Bo WEI, Xin YU, Bing LIU, Zhuoying WU, Li GU

Frontiers of Environmental Science & Engineering 2010, Volume 4, Issue 4,   Pages 459-465 doi: 10.1007/s11783-010-0249-3

Abstract: The biologic activated carbon (BAC) process is widely used in drinking water treatments.However, the bottleneck of deoxyribonucleic acid (DNA) extraction from BAC attached biofilm has to beconventional procedure was unsuccessful due to firm biomass attachment and adsorption capacity of the BACThis protocol was further tested using the vertical BAC samples from the full-scale biofilter of PinghuThe results showed the DNA yielded a range of 40 μg·g BAC (dry weight) to over 100 μg·g BAC (dry weight

Keywords： bacterial DNA extraction     biological activated carbon (BAC)     biofilm     water treatment     pretreatment protocol    

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High-expression of recombinant human concensus interferon-α by Pichia pastoris

HAO Yuyou, SHI Qiqi, HE Yun, ZHUANG Yingping, WANG Yonghong, ZHANG Siliang, CHU Ju, LIU Zhimin

Frontiers of Chemical Science and Engineering 2007, Volume 1, Issue 4,   Pages 399-403 doi: 10.1007/s11705-007-0073-x

Abstract: The present work focused on the high expression of recombinant human consensus interferon- (cIFN) by

Keywords： bioactivity     pastoris     maximum     induction     optimized    

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Expression and function of DMT1 without IRE in C6 cells mediated by recombinant adenovirus

Xixun DU*, Huamin XU*, Hong JIANG, Jun WANG, Lei WANG, Junxia XIE

Frontiers of Medicine 2009, Volume 3, Issue 1,   Pages 67-71 doi: 10.1007/s11684-009-0010-0

Abstract: In the present study, we constructed a recombinant adenovirus containing the gene of DMT1 without theIRE was then transfected into E1-transformed human embryonic kidney cells (HEK293 cells) , in which recombinantPCR confirmed pAdEasy1-DMT1-IRE contained gene DMT1-IRE, indicating the successful construction of recombinant

Keywords： divalent metal transporter 1     recombinant adenovirus     homologous recombination     iron    

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Recombinant protein diannexin prevents preeclampsia-like symptoms in a pregnant mouse model via reducing

Frontiers of Medicine 2022, Volume 16, Issue 6,   Pages 919-931 doi: 10.1007/s11684-021-0918-6

Abstract: This study describes a strategy to prevent PE by reducing MP release using the recombinant protein, diannexin

Keywords： preeclampsia     recombinant protein diannexin     microparticle     NLRP3 inflammasome     phosphatidylserin    

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Clinical evaluation of recombinant human growth hormone injection in children with growth hormone deficiency

Ling HOU, Xiaoping LUO, Minlian DU, Huamei MA, Chunxiu GONG, Yuchuan LI, Shuixian SHEN, Zhuhui ZHAO, Li LIANG, Guanping DONG, Chaoying YAN, Hongwei DU

Frontiers of Medicine 2009, Volume 3, Issue 2,   Pages 171-176 doi: 10.1007/s11684-009-0027-4

Abstract: Recombinant human growth hormone (rhGH) has been widely used in the clinical treatment of growth hormone

Keywords： recombinant human growth hormone     injection     growth hormone deficiency    

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supplementation of thyroid hormone on treatment of idiopathic short-stature children during therapy with recombinant

Wei Wang, Shuqin Jiang, Zhirui Cui, Xiangyang Luo, Lingli Shi, Heli Zheng

Frontiers of Medicine 2018, Volume 12, Issue 5,   Pages 580-585 doi: 10.1007/s11684-017-0585-9

Abstract: deficiency on growth rate should be considered when FT4 level lies in the low-normal range prior to recombinant

Keywords： therapeutic     idiopathic short-stature children     free T4     the first year     recombinant human growth hormone    

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High-level expression of recombinant IgG1 by CHO K1 platform

Ningning Xu, Jianfa Ou, Al-Karim (Al) Gilani, Lufang Zhou, Margaret Liu

Frontiers of Chemical Science and Engineering 2015, Volume 9, Issue 3,   Pages 376-380 doi: 10.1007/s11705-015-1531-5

Abstract: The Chinese Hamster Ovary (CHO K1) cell was used to express a targeted anti-cancer monoclonal antibody by optimizing the platform of the construction of production cell line in this study. The adherent CHO K1 was first adapted to suspension culture in chemical defined medium. Then the glutamine synthetase (GS) vector was applied to construct a single plasmid to overexpress a monoclonal antibody IgG1. Post transfection, the production of cell pool was optimized by glutamine-free selection and amplification using various concentrations of methionine sulfoximine. The best cell pool of CHO K1/IgG1 was used to screen the top single clone using the limiting dilution cloning. Finally, a high IgG1 production of 780 mg/L was obtained from a batch culture. This study demonstrated that the construction of high producing cell line, from gene to clone, could be completed within six month and the gene amplification improved protein production greatly.

Keywords： Chinese hamster ovary (CHO)     monoclonal antibody     IgG1     amplification     cell line development    

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Regulation of exogenous bFGF gene mediated by recombinant adeno-associated virus

Ke SONG, Nianjing RAO, Meiling CHEN, Yingguang CAO

Frontiers of Medicine 2009, Volume 3, Issue 2,   Pages 158-163 doi: 10.1007/s11684-009-0042-5

Abstract: The regulatory effect of basic fibroblast growth factor (bFGF) mediated by recombinant adeno-associatedRecombinant plasmid pAAV-S3-bFGF, and pSVneo were co-transfected into BHK-21 cells, then the recombinantThe titer of the recombinant rAAV2-tet-off-bFGF was determined by dot-blot assay.It was concluded that rAAV2-tet-off-bFGF could infect MC3T3 cells efficiently, and this recombinant system

Keywords： tetracycline regulatory system     adeno-associated virus     basic fibroblast growth factor     gene regulation    

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Study of recombinant human IFN-α-2b bacilli Calmette–Guerin activated killer cells and against bladder

FAN Xiaodong, HAN Ruifa

Frontiers of Medicine 2007, Volume 1, Issue 4,   Pages 377-380 doi: 10.1007/s11684-007-0073-8

Abstract: Therefore, current studies aim at developing recombinant BCG (rBCG) strains secreting Th1-like cytokinesRecombinant IFN--2b-BCG, wild-type BCG (wBCG), wild-type BCG and IFN--2b were coincubated with PBMCsThe result of MTT showed that the proliferation of PBMC in the recombinant BCG group was more powerfulThe result of LDH release assay showed that the antitumor activity of BAK cells stimulated by RecombinantWe conclude that the recombinant BCG can activate more PBMCs to anti-bladder cancer than wild-type BCG

Keywords： Urology     powerful     2b-BCG     Tianjin institute     wild-type    

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A review of optimization modeling and solution methods in renewable energy systems

Frontiers of Engineering Management   Pages 640-671 doi: 10.1007/s42524-023-0271-3

Abstract: Science database, this study reviews the decision-making optimization problems, models, and solution methods

Keywords： renewable energy system     bibliometrics     mathematical programming     optimization models     solution methods    

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Regulatory issues for genetically modified animals

Perry Bradbury HACKETT

Frontiers of Agricultural Science and Engineering 2020, Volume 7, Issue 2,   Pages 188-203 doi: 10.15302/J-FASE-2019307

Abstract:

Precision genetics and breeding have the potential to meet the agricultural needs and goals of the world in the 21st century. These needs include increasing the efficiency of production of animals and improving their products with minimal impact on the environment. The USA is the major innovator in genomic science and the acknowledged leader in formulating policies to regulate genetic applications in medicine and agriculture. However, governments worldwide have been exceedingly reluctant to support the introduction of genetically modified (GM) animals into agriculture. Regulatory policies have stagnated due to legal guidelines that could not anticipate the needs and solutions that are evident today. This must change if we are to maintain planetary integrity. I propose a new, market-based regulatory model for GM livestock that has both a strong scientific foundation and has worked for 10000 years. The model is similar to that for information technology in which specific algorithms drive computer and cell phone applications. Genome engineers write genetic algorithms that drive the traits in biological organisms. Accordingly, GM products should be viewed in terms of their use and public benefit rather than by limitations to the genetic programing coming from a few highly vocal groups. Genetic algorithms (Genapps) of the 21st century will include not only introduction of synthetic genes, but also complete natural and synthetic biochemical pathways to produce agricultural products that are maximally efficient, healthy to humans and animals, and sustainable in an era of changing climates while avoiding environmental degradation.

Keywords： algorithms     editing     FDA     GMO     recombinant DNA     USDA    

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Antifungal and antibacterial functions of medicinal leech recombinant destabilase-lysozyme and its heated-up

T. G. YUDINA, Danyang GUO, N. F. PISKUNKOVA, I. B. PAVLOVA, L. L. ZAVALOVA, I. P. BASKOVA

Frontiers of Chemical Science and Engineering 2012, Volume 6, Issue 2,   Pages 203-209 doi: 10.1007/s11705-012-1277-2

Abstract: Antifungal activity of recombinant medicinal leech destabilase-lysozyme (rec.Dest-Lys) was investigated

Keywords： recombinant destabilase-lysozyme (rec.Dest-Lys)     antimicrobial activity     antifungal activity     medicinal    

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Intelligent methods for the process parameter determination of plastic injection molding

Huang GAO, Yun ZHANG, Xundao ZHOU, Dequn LI

Frontiers of Mechanical Engineering 2018, Volume 13, Issue 1,   Pages 85-95 doi: 10.1007/s11465-018-0491-0

Abstract:

Injection molding is one of the most widely used material processing methods in producing plasticThis article reviews the recent studies and developments of the intelligent methods applied in the processThese intelligent methods are classified into three categories: Case-based reasoning methods, expertsystem-based methods, and data fitting and optimization methods.

Keywords： injection molding     intelligent methods     process parameters     optimization    

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Title Author Date Type Operation

Construction of a universal recombinant expression vector that regulates the expression of human lysozyme

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Journal Article

Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Journal Article

Development of pretreatment protocol for DNA extraction from biofilm attached to biologic activated carbon (BAC

Shuting ZHANG, Bo WEI, Xin YU, Bing LIU, Zhuoying WU, Li GU

Journal Article

High-expression of recombinant human concensus interferon-α by Pichia pastoris

HAO Yuyou, SHI Qiqi, HE Yun, ZHUANG Yingping, WANG Yonghong, ZHANG Siliang, CHU Ju, LIU Zhimin

Journal Article

Expression and function of DMT1 without IRE in C6 cells mediated by recombinant adenovirus

Xixun DU*, Huamin XU*, Hong JIANG, Jun WANG, Lei WANG, Junxia XIE

Journal Article

Recombinant protein diannexin prevents preeclampsia-like symptoms in a pregnant mouse model via reducing

Journal Article

Clinical evaluation of recombinant human growth hormone injection in children with growth hormone deficiency

Ling HOU, Xiaoping LUO, Minlian DU, Huamei MA, Chunxiu GONG, Yuchuan LI, Shuixian SHEN, Zhuhui ZHAO, Li LIANG, Guanping DONG, Chaoying YAN, Hongwei DU

Journal Article

supplementation of thyroid hormone on treatment of idiopathic short-stature children during therapy with recombinant

Wei Wang, Shuqin Jiang, Zhirui Cui, Xiangyang Luo, Lingli Shi, Heli Zheng

Journal Article

High-level expression of recombinant IgG1 by CHO K1 platform

Ningning Xu, Jianfa Ou, Al-Karim (Al) Gilani, Lufang Zhou, Margaret Liu

Journal Article

Regulation of exogenous bFGF gene mediated by recombinant adeno-associated virus

Ke SONG, Nianjing RAO, Meiling CHEN, Yingguang CAO

Journal Article

Study of recombinant human IFN-α-2b bacilli Calmette–Guerin activated killer cells and against bladder

FAN Xiaodong, HAN Ruifa

Journal Article

A review of optimization modeling and solution methods in renewable energy systems

Journal Article

Regulatory issues for genetically modified animals

Perry Bradbury HACKETT

Journal Article

Antifungal and antibacterial functions of medicinal leech recombinant destabilase-lysozyme and its heated-up

T. G. YUDINA, Danyang GUO, N. F. PISKUNKOVA, I. B. PAVLOVA, L. L. ZAVALOVA, I. P. BASKOVA

Journal Article

Intelligent methods for the process parameter determination of plastic injection molding

Huang GAO, Yun ZHANG, Xundao ZHOU, Dequn LI

Journal Article